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atg7  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc atg7
    Atg7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atg7/pm41950919-610-22-23
    Average 86 stars, based on 1 article reviews
    atg7 - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    other:

    Article Title: Copper potentiates PUFA-mediated antitumor activity by activating Lipophagy.
    Article Snippet: Polyunsaturated fatty acids (PUFAs) have demonstrated promising anticancer properties by inducing cancer cell death and inhibiting cancer metastasis.. As dynamic organelles, lipid droplets (LDs) may protect cells from PUFAinduced lipotoxicity by sequestering excess fatty acids.. However, the underlying mechanisms regulating LDs dynamics in PUFA-mediated tumor cytotoxicity remain poorly understood.

    Western Blot:

    Article Title: Effect of reticulocalbin 3 on monocyte-to-macrophage differentiation in sepsis through modulating autophagy
    Article Snippet: .. The samples were subjected to Western blotting with specific antibodies against RCN3 (Sigma, #HPA050402), LC3B (NOVUS, #NB100-2220, USA), and Atg7 (Cell Signaling Technology, #2631, USA). ..

    Article Title: Geniposide ameliorates ferroptosis through the ROS/p38 MAPK/NCOA4/GPX4-mediated ferritinophagy in a co-culture system of HMC3 and SH-SY5Y cells.
    Article Snippet: .. The antibodies used for immunoblotting were as follows: LC3B, p62, Atg7, p-p38 MAPK, total p38 MAPK, p-ERK, total ERK, p-JNK, total JNK, HO-1, p-NFκB, total NF-κB, Iba-1, SQSTM1/p62, LC3B, Atg5, Beclin-1, (Cell Signaling Technology, Danvers, MA); TNF-α, IL-1β (Santa Cruz Biotechnology Inc., Santa Cruz, TX); β-actin (Sigma-Aldrich Inc., St. Louis, MO); COX-2, BDNF, LAMP2, NCOA4, GPX4, FTH1, Transferrin, xCT (Abcam, Waltham, MA). .. Afterward, membranes were incubated with the secondary antibody for 60 min. Immunoreactive proteins were visualized using a chemiluminescent substrate (Amersham Life Sciences Inc., Arlington Heights, IL).

    Incubation:

    Article Title: Species-specific cleavage of the autophagy adaptor p62 dictates responses to TNF.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER HALO image analysis platform v3.6.4134.263 Indica Labs https://indicalab.com/halo/ Area Quantification v2.4.2 algorithm Indica Labs https://indicalab.com/halo/ CytoNuclear v2.0.9 algorithm (HALO) Indica Labs https://indicalab.com/halo/ CRISPOR Concordet et al.59 https://crispor.gi.ucsc.edu Image Lab Touch Software v. 2.4.0.03 BioRad https://www.bio-rad.com ImageJ64 software ImageJ https://imagej.net/ij/ Incucyte Software v. 2024B Sartorius https://www.sartorius.com/en/products/ live-cell-imaging-analysis/live-cellanalysis-software/incucyte-softwarev2024b Other Orbitrap FusionTM LumosTM TribridTM Mass Spectrometer Thermo Fisher Scientific Cat# IQLAAEGAAPFADBMBHQ Easy-nLC 1200 LC System Thermo Fisher Scientific Cat# LC140 50 cm Emitters CoAnn Technologies Cat# ICT36007508-50 ABIRD - The Active Background Ion Reduction Device for nanospray LC/MS ESI Solutions Cat# ABIRD ll OPEN ACCESS Article e5 Molecular Cell 86, 1–13.e1–e11, April 16, 2026 membranes (Merck). .. Membranes were blocked with BSA 5% in TBST for 30 min and incubated with the following primary antibodies overnight (all 1:1000 dilution): ATG7 (Cell Signaling, Cat# 8558; RRID:AB_11179039), BiP (Cell Signaling, Cat# 3177; RRID:AB_2119845), β actin (Cell Signaling, Cat# 4970; RRID:AB_2223172), Caspase-2 (BD Transduction Laboratories, Cat# 611023; RRID:AB_397606), Caspase-8 (Cell Signaling, Cat# 4790; RRID:AB_10545723), Caspase-9 (Cell Signaling, Cat# 9502; RRID:AB_2228052), CHOP (Cell Signaling, Cat# 2895; RRID:AB_2089254), Cleaved caspase-3 (Asp175) (Cell Signaling, Cat# 9664; RRID:AB_2070042), Cleaved caspase 7 (Asp198) (Cell Signaling, Cat# 9491; RRID:AB_2068261), Cleaved caspase-8 (Cell Signaling, Cat# 9429; RRID:AB_2062634), Cleaved caspase-8 (Asp374) (for human samples) (Cell Signaling, Cat# 9496S; RRID:AB_561378), Cleaved caspase-8 (mouse specific) (Cell Signaling, Cat# 8592S; RRID:AB_10999739), Cleaved PARP (Asp214) (rodent specific) (Cell Signaling, Cat# 94885; RRID:AB_2800237), FADD (Abcam, Cat# 124812; RRID:AB_11155370), FADD (Merck, Cat# 05-486; RRID:AB_309984), FLAG (Sigma-Aldrich, Cat# F7425; RRID:AB_439687), FLAG (M2) mAb (Merck, Cat# F3165; RRID:AB_2209044), GAPDH (Abcam, Cat# ab9484; RRID:AB_307274), GFP (Santa Cruz Biotechnology, Cat# sc-9996; RRID:AB_627695), HA-tag (Merck, Cat# 11867423001; RRID:AB_390918), HSP90 (Santa Cruz, Cat# sc-1057; RRID:AB_675714), LC3B (Cell Signaling, Cat# 3868; RRID:AB_2137707), MLKL (Cell Signaling, Cat# 14993; RRID:AB_2687572), p62 in human cells (BD Transduction Laboratories, Cat# 610833; RRID:AB_398152), p62 in mouse cells (Sigma-Aldrich, Cat# P0067; RRID:AB_1841028), p-MLKL (Cell Signaling, Cat# 91689; RRID:AB_2732034), pRIPK1 S166 (rodent specific) (Cell Signaling, Cat# 31122; RRID:AB_2799000), p-RIPK1 (Ser166) (Cell Signaling, Cat# 65746; RRID:AB_2799693), RIPK1 (BD Transduction Laboratories, Cat# 610459; RRID:AB_398668), TRADD (Abcam, Cat# ab319029; RRID: AB_3741122), TRADD (Cell Signaling, Cat# 3694; RRID:AB_2209044). .. Vinculin (Sigma-Aldrich, Cat# V9131; RRID:AB_477629).

    Article Title: Structure-based discovery of a novel allosteric activator of ATG4B for the treatment of triple-negative breast cancer.
    Article Snippet: Triple-negative breast cancer (TNBC) is highly malignant and lacks effective therapeutic drugs.. ATG4B, a critical cysteine protease in autophagy, regulates LC3B processing and recycling to promote autophagosome formation, and its activation represents a promising therapeutic strategy for cancer.. Based on structure-based drug design, this study conducted virtual screening of the SPECS compound library and optimized the structure through structure-activity relationship analysis, obtaining a novel ATG4B allosteric activator, 16a.

    Membrane:

    Article Title: Structure-based discovery of a novel allosteric activator of ATG4B for the treatment of triple-negative breast cancer.
    Article Snippet: Triple-negative breast cancer (TNBC) is highly malignant and lacks effective therapeutic drugs.. ATG4B, a critical cysteine protease in autophagy, regulates LC3B processing and recycling to promote autophagosome formation, and its activation represents a promising therapeutic strategy for cancer.. Based on structure-based drug design, this study conducted virtual screening of the SPECS compound library and optimized the structure through structure-activity relationship analysis, obtaining a novel ATG4B allosteric activator, 16a.

    Blocking Assay:

    Article Title: Structure-based discovery of a novel allosteric activator of ATG4B for the treatment of triple-negative breast cancer.
    Article Snippet: Triple-negative breast cancer (TNBC) is highly malignant and lacks effective therapeutic drugs.. ATG4B, a critical cysteine protease in autophagy, regulates LC3B processing and recycling to promote autophagosome formation, and its activation represents a promising therapeutic strategy for cancer.. Based on structure-based drug design, this study conducted virtual screening of the SPECS compound library and optimized the structure through structure-activity relationship analysis, obtaining a novel ATG4B allosteric activator, 16a.



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